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th17 cell polarization medium  (R&D Systems)


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    R&D Systems th17 cell polarization medium
    Th17 Cell Polarization Medium, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flowx+human+th17+cell+multi+color+flow+cytometry+kit/pm40089498-394-0-9?v=R%26D+Systems
    Average 93 stars, based on 11 article reviews
    th17 cell polarization medium - by Bioz Stars, 2026-07
    93/100 stars

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    a. Schematic of the Th17 and iTreg differentiation protocol for primary human naïve CD4 + CD25 - T cells isolated from the umbilical cord blood of healthy neonates. CD4 + CD25 - T cells were activated with anti-CD3/anti-CD28 and differentiated into Th17 or iTreg cells in the presence of corresponding cytokines for 3 days. DMSO control or conjugated bile acids (Asn-UDCA, Ser-CDCA, Tyr-CDCA, and unconjugated UDCA) at 100 µM were added on day 0 of differentiation. b-e. IL-17a secretion in the supernatant of Th17 cultures treated with Asn-UDCA ( b ), Ser-CDCA ( c ), Tyr-CDCA ( d ), and UDCA ( e ) was quantified on day 3 of differentiation from four biological replicates using ELISA. f-i. Intracellular Foxp3 protein expression in iTregs cultured with Asn-UDCA ( f ), Ser-CDCA ( g ), Tyr-CDCA ( h ), or UDCA ( i ) was assessed on day 3 of differentiation by flow cytometry. Geometric mean fluorescence intensity (MFI) values are shown for four biological replicates. Statistical significance was determined using paired, two-tailed Student’s t-test.

    Journal: medRxiv

    Article Title: Trajectories of microbiome-derived bile acids in early life – insights into the progression to islet autoimmunity

    doi: 10.1101/2025.02.18.25322275

    Figure Lengend Snippet: a. Schematic of the Th17 and iTreg differentiation protocol for primary human naïve CD4 + CD25 - T cells isolated from the umbilical cord blood of healthy neonates. CD4 + CD25 - T cells were activated with anti-CD3/anti-CD28 and differentiated into Th17 or iTreg cells in the presence of corresponding cytokines for 3 days. DMSO control or conjugated bile acids (Asn-UDCA, Ser-CDCA, Tyr-CDCA, and unconjugated UDCA) at 100 µM were added on day 0 of differentiation. b-e. IL-17a secretion in the supernatant of Th17 cultures treated with Asn-UDCA ( b ), Ser-CDCA ( c ), Tyr-CDCA ( d ), and UDCA ( e ) was quantified on day 3 of differentiation from four biological replicates using ELISA. f-i. Intracellular Foxp3 protein expression in iTregs cultured with Asn-UDCA ( f ), Ser-CDCA ( g ), Tyr-CDCA ( h ), or UDCA ( i ) was assessed on day 3 of differentiation by flow cytometry. Geometric mean fluorescence intensity (MFI) values are shown for four biological replicates. Statistical significance was determined using paired, two-tailed Student’s t-test.

    Article Snippet: After differentiation, secreted IL-17a levels were determined from Th17 cell-culture supernatants at 72 hours using the human IL-17a DuoSet ELISA kit (R&D Systems, Cat# DY317-05, DY008).

    Techniques: Isolation, Control, Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture, Flow Cytometry, Fluorescence, Two Tailed Test